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Registro Completo |
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Biblioteca(s): |
Biblioteca Rui Tendinha. |
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Data corrente: |
08/01/2014 |
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Data da última atualização: |
01/09/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
ABREU, P. M. V.; PICCIN, J. G.; RODRIGUES, S. P.; VENTURA, J. A.; FERNANDES, P. M. B. |
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Afiliação: |
Paolla M.V. Abreu, UFES; João G. Piccin, UFES; Silas P. Rodrigues, UFES; Jose Aires Ventura, Incaper; Patricia M.B. Fernandes, UFES. |
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Título: |
Molecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Journal of Virological Methods, Amsterdam-Holanda, v. 180, p. 11-17, 2012. |
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Páginas: |
7p. |
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ISSN: |
0166-0934 |
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DOI: |
10.1016/j.jviromet.2011.12.003 |
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Idioma: |
Português |
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Conteúdo: |
Papaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed. |
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Palavras-Chave: |
DsRNA; Papaya; Papaya meleira virus; Phytopathology; Real-time RT-PCR; Resistance induction. |
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Categoria do assunto: |
-- |
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URL: |
http://ac.els-cdn.com/S0166093411004885/1-s2.0-S0166093411004885-main.pdf?_tid=597935f2-7857-11e3-a060-00000aacb35e&acdnat=1389180411_31ada3a608c8d17c0a26fd345cb062ae
http://biblioteca.incaper.es.gov.br/digital/bitstream/item/162/1/JVirolMethods-2012.PDF
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Marc: |
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022 $a0166-0934
024 7 $a10.1016/j.jviromet.2011.12.003$2DOI
100 1 $aABREU, P. M. V.
245 $aMolecular diagnosis of Papaya meleira virus (PMeV) from leaf samples of Carica papaya L. using conventional and real-time RT-PCR.$h[electronic resource]
260 $c2012
300 $a7p.
520 $aPapaya meleira virus (PMeV) is the causal agent of papaya sticky disease. This study describes two methods for molecular diagnosis of PMeV using conventional and real-time PCR. These methods were shown to be more efficient than current methods of viral detection using extraction of PMeV dsRNA and observation of symptoms in the field. The methods described here were used to evaluate the effect of inoculation of papaya plants with purified PMeV dsRNA on the progress of PMeV infection. A single inoculation with PMeV dsRNA was observed to delay the progress of the virus infection by several weeks. The possibility of vertical transmission of PMeV was also investigated. No evidence was found for PMeV transmission through seeds collected from diseased fruit. The implications of these results for the epidemiology of PMeV and the management of papaya sticky disease are discussed.
653 $aDsRNA
653 $aPapaya
653 $aPapaya meleira virus
653 $aPhytopathology
653 $aReal-time RT-PCR
653 $aResistance induction
700 1 $aPICCIN, J. G.
700 1 $aRODRIGUES, S. P.
700 1 $aVENTURA, J. A.
700 1 $aFERNANDES, P. M. B.
773 $tJournal of Virological Methods, Amsterdam-Holanda$gv. 180, p. 11-17, 2012.
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Registro original: |
Biblioteca Rui Tendinha (BRT) |
